Italy - Venice, Florence, Siena, Rome

We are going to do a land tour of Italy before an Italy/Greece cruise.  We will have a few days each in Venice, Florence, Siena and Rome.  We've done the Vatican and the Colosseum in Rome but haven't been to the other places.  There are some obvious sites to see such as the Forum and key museums in each city.  I am interested if anyone has some ideas on things to do that are not the obvious key things to do in any of these cities.

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